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We should always remember that following antibiotics inhibit the protein synthesis e.g.anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, puromycin etc.
The cistron DNA is transcribed into a variety of RNA intermediates.
The last version is used as a template in synthesis of a polypeptide chain.
When the t RNA has an amino acid linked to it, it is termed "charged".
Initiation involves the small subunit of the ribosome binding to 5' end of m RNA with the help of initiation factors (IF), other proteins that assist the process.
A proprotein is an inactive protein containing one or more inhibitory peptides that can be activated when the inhibitory sequence is removed by proteolysis during posttranslational modification.
A preprotein is a form that contains a signal sequence (an N-terminal signal peptide) that specifies its insertion into or through membranes, i.e., targets them for secretion.
In translation, messenger RNA (m RNA) is decoded to produce a specific polypeptide according to the rules specified by the trinucleotide genetic code.
This uses an m RNA sequence as a template to guide the synthesis of a chain of amino acids that form a protein.
While this is not technically a step in translation, it is required for translation to proceed.
The AA is joined by its carboxyl group to the 3' OH of the t RNA by an ester bond.